Wednesday, 10 September 2014

Difference between vegetative cells & Endospore

            



                     
s.no
Characterstic
Vegetative cell
Endospore
1
Structure
 Typical gram positive cell ;       Few gram negative cells
Thick spore cortex, spore coat & Exosporium 
2
Microscopic appearance
 Non-refractile
Refractile 
3
Calcium content
 Low
high 
4
Dipiclonic acid
 Absent
Present 
5
Enzyme activity
 High
Low 
6
Metabolism(O2 uptake)
 High
Low or absent 
7
Macromolecular synthesis
 Present
Absent 
8
mRNA
 Present
 Low or absent 
9
Heat resistance
 Low 
High
10
Radiation resistance
 Low 
 High
11
Resistance to chemicals(for e.g. H2O2) & acids
 Low 
 High
12
Stainability by dyes
 Stainable
Stainable only with specific method 
13
Action of lysosomes
 Sensitive
Resistance 
14
Water content
 High, 80-90%
 Low, 10-25% in core
15
Small acid-soluble protein
Absent 
Present 
16
Cytoplasmic PH
 Above PH 7
 Above PH 5.5-6.0 in core

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