Campylobacter infections are
among the most common bacterial infections in humans. They produce both
diarrheal and systemic illnesses. In industrialized regions, enteric Campylobacter infections
produce an inflammatory, sometimes bloody, diarrhea or dysentery syndrome.
•
Campylobacter jejuni is the leading cause of
gastroenteritis in the US and probably world-wide.
•
Hawai'i has the highest incidence in the
country à about
900 reported cases a year with an incidence of 75/100,000, but the thinking is
that infections are grossly under reported.
History of Campylobacter jejuni
Awareness of the public health
implications of Campylobacter infections
has evolved over more than a century. In 1886, Escherich observed organisms
resembling Campylobacters in stool samples of children with diarrhea. In 1913, McFaydean and
Stockman identified Campylobacters (called related Vibrio) in fetal tissues of aborted sheep. In 1957,
King described the isolation of related Vibrio from blood samples of children
with diarrhea, and in 1972, clinical microbiologists in Belgium first isolated Campylobacters from stool samples of patients with diarrhea. The development of
selective growth media in the 1970s permitted more laboratories to test stool
specimens for Campylobacter. Soon Campylobacter [species]
were established as common human pathogens.
Properties of the organism
•
Curved s-shaped gram (-) rods, motile with a
single polar flagellum at one or both ends.
•
Defined "viable but not culturable"
state.
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Respiratory metabolism, microaerophilic.
•
Grow with 10% CO2 / 5% O2. Some species / strains require additional H2
in the atmosphere
•
C. jejuni will grow at 42o C and
this is used as a selection criterion.
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The organism is unusually thin (0.2 - 0.9m).
•
Viable but non culturable
Reservoirs and epidemiology
Human cases are associated with:
•
Poultry - especially eating chicken out
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Pets - especially young puppies
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Water supply
•
Raw milk
•
Most cases occur in the summer months -- late
spring to early autumn.
Cell Structure and Metabolism
Campylobacter, a curved and motile rod, is often flagellated and
causes bacterial infections in the body (FDA, 2003). Gene sets have been
identified that produce sialic acid on the cell surface. This sialic acid
enables Campylobacter to survive in the host's body by avoiding the
body's immune response, sometimes even confusing the body into attacking itself
(Sanger Institute).
Pathogenesis and Disease Characteristics
•
Low infectious dose
•
Two disease entities:
v Diarrhea
v Dysentery
•
Associated with Guillain-Barre syndrome.
Virulence factors
•
C.jejuni can invade intestinal epithelial
cells.
•
C.jejuni secretes a number of novel proteins
upon cultivation with enterocytes:
v CiaB
•
pVir, present only in some strains of Cj
appears to be important for invasion.
•
Microtubule mediated endocytosis occurs
•
Cj apparently stays within vacuole
•
Adenyl cyclase activating cholera toxin-like
enterotoxin
•
Microtubule mediated endocytosis occurs and
microfilament mechanism may be involved too
•
Microtubules aggregate into finger-like
protrusions with C.jejuni at the tips.
•
Cytolethal Distending Toxin
v Irreversible
cell cycle arrest
v All
three CDT genes need to be expressed for activity
•
Adenyl cyclase activating enterotoxin?
Guillaine-Barrè Syndrome
•
Ascending muscle weakness or paralysis, rapidly
progressing.
•
40% of GBS patients have evidence of
Campylobacter infection.
•
LPS oligosaccharides structurally related to
human motor neuron gangliosides.
Precautions to C.jejuni infections
·
Keep raw meat, especially poultry,
separate from other foods.
·
Do not drink raw or unpasteurized
milk.
Campylobacter infection treatment
The use of antibiotics to
treat Campylobacter infections is controversial, with studies
showing that erythromycin rapidly eliminated Campylobacter organisms
from the stool without affecting the duration of illness. Studies in children
with C jejuni dysentery have shown benefit from early
treatment with erythromycin. Antibiotics may be indicated if any of the
following occur:
- High
fever
- Bloody
diarrhea
- Excessive
bowel movements (ie, >8 stools per day)
- Worsening
symptoms
- Failure
of symptoms to improve
- Persistence
of symptoms for longer than 1 week
- Pregnancy
- HIV
infection and other immunocompromised states
- Avoid
antimotility agents because they prolong the duration of symptoms and have
been associated with fatalities.
- Individuals
with hypogammaglobulinemia who have recurrent C jejunibacteremia
may require fresh frozen plasma with antibiotics.
- Patients
with severe dysentery or a relapsing course may require hospitalization.
- Patients
with endovascular C fetus infections require at least 4
weeks of treatment; gentamicin is believed to be the agent of choice.
Treatment with ampicillin or third-generation cephalosporins is an
alternative.
- Treat C
fetus CNS infections for 2-3 weeks with third-generation
cephalosporins, ampicillin, or chloramphenicol.
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